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目的:观察Fra-1对C6胶质瘤侵袭转移能力的影响。方法:以大鼠C6胶质瘤细胞为研究对象,Fra-1 siRNA通过脂质体转染C6,realtime RT-PCR和western blot法检测C6细胞中Fra-1的表达、Elisa法检测细胞培养上清中MMP-9的含量,Transwell小室观察C6细胞的转移侵袭能力。结果:干扰Fra-l后能降低C6细胞中Fra-1的表达,显著降低C6细胞中MMP-9的含量,降低C6细胞的转移侵袭能力。结论:干扰Fra-1能抑制C6细胞的转移侵袭。
Objective: To observe the effect of Fra-1 on invasion and metastasis of C6 glioma. Methods: C6 glioma cells were used as experimental materials. Fra-1 siRNA was transfected into C6 cells by lipofectamine. Real-time RT-PCR and western blot were used to detect the expression of Fra-1 in C6 cells. Elisa assay was used to detect cell proliferation Clear MMP-9 content, Transwell chamber observation of C6 cell migration and invasion. Results: The interference of Fra-1 could decrease the expression of Fra-1 in C6 cells, significantly decrease the content of MMP-9 in C6 cells and decrease the invasion and metastasis of C6 cells. Conclusion: Interference with Fra-1 can inhibit the invasion and metastasis of C6 cells.