论文部分内容阅读
本研究通过农杆菌介导法,获得了5个PCR和Southern杂交检测阳性的AtHDG11转基因黑麦草株系。相比野生型再生植株,转基因植株具有更发达的根系,同时在干旱胁迫下具有较低的MDA含量,并且具有较高水平的超氧化物歧化酶(superoxide dismutase,SOD)和过氧化氢酶(catalase,CAT)活性。这些结果均显示过量表达AtHDG11能够提高黑麦草的耐旱性。
In this study, five transgenic lines of AtHDG11 transgenic ryegrass were obtained by Agrobacterium tumefaciens-mediated method. Compared with the wild-type regenerated plants, the transgenic plants had a more developed root system with lower MDA content under drought stress and a higher level of superoxide dismutase (SOD) and catalase catalase, CAT) activity. These results show that overexpression of AtHDG11 can improve the drought tolerance of ryegrass.