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目的探讨丹参酮ⅡA(TanⅡA)对局灶性脑缺血再灌注损伤大鼠脑组织单核细胞趋化蛋白-1(MCP-1)和白细胞介素-1β(IL-1β)含量的影响。方法将大鼠随机分为假手术组、缺血再灌注组、TanⅡA低剂量治疗组和TanⅡA高剂量治疗组,线栓法建立局灶性脑缺血再灌注模型。TanⅡA高、低剂量治疗组分别于术前连续灌胃给予高、低剂量TanⅡA3d,每日1次。各组于脑缺血90min再灌注24h进行2,3,5-三苯基氯化四氮唑染色观察脑梗死体积和脑含水量检测,采用酶联免疫吸附试验法检测脑组织MCP-1和IL-1β含量的变化。结果TanⅡA治疗组脑梗死体积较缺血再灌注组减小,脑含水量较缺血再灌注组减少,而高、低剂量组之间差异亦具有显著性。与假手术组比较,缺血再灌注组脑组织MCP-1和IL-1β含量明显升高;与缺血再灌注组比较,TanⅡA高、低剂量治疗组脑组织MCP-l和IL-1β含量均明显降低,高、低剂量组之间差异具有显著性。结论降低缺血再灌注损伤脑组织MCP-1和IL-1β含量,抑制再灌注损伤炎症反应,可能是TanⅡA发挥脑保护的重要途径之一。
Objective To investigate the effect of tanshinone IIA (Tan IIA) on the content of monocyte chemoattractant protein-1 (MCP-1) and interleukin-1β (IL-1β) in rats with focal cerebral ischemia-reperfusion injury. Methods Rats were randomly divided into sham operation group, ischemia reperfusion group, TanIIA low dose treatment group and TanIIA high dose treatment group. The model of focal cerebral ischemia reperfusion was established by suture. TanIIA high-dose and low-dose treatment groups were given intragastric administration of high and low doses of TanIIA3d once daily before surgery. The cerebral infarct volume and brain water content were measured by 2,3,5-triphenyltetrazolium staining in each group at 90 min after cerebral ischemia and 24 h after reperfusion. The brain tissue MCP-1 was detected by enzyme-linked immunosorbent assay. Changes in IL-1β content. Results The volume of cerebral infarction in TanIIA group was smaller than that in ischemia-reperfusion group, and the brain water content was lower than that in ischemia-reperfusion group. The difference between high and low dose groups was also significant. Compared with the sham-operated group, the contents of MCP-1 and IL-1β in the brain tissue of the ischemia-reperfusion group were significantly increased. Compared with the ischemia-reperfusion group, the contents of MCP-1 and IL-1β in the brain tissue of the high- and low-dose Tan IIA group were significantly increased. Significantly decreased, the difference between high and low dose groups was significant. Conclusion Decreasing the content of MCP-1 and IL-1β in brain tissue of ischemia-reperfusion injury and inhibiting the inflammatory response to reperfusion injury may be one of the important ways for TanIIA to exert brain protection.