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目的构建表达小鼠CD1_D基因和B7-1基因的真核表达载体。方法用PCR方法获得基因,定向克隆连接到真核表达载体上,得到重组真核表达载体,使用酶切方法加以鉴定。结果酶切得到的片段与所需相符,得到重组的逆转录病毒载体。结论重组小鼠CD1_D基因和B7-1基因真核表达载体的构建为今后进一步的研究奠定了基础。
Objective To construct eukaryotic expression vectors expressing mouse CD1_D and B7-1 genes. Methods The gene was obtained by PCR and cloned into eukaryotic expression vector. The recombinant eukaryotic expression vector was obtained and identified by restriction enzyme digestion. Results The digested fragments were matched to the requirements, resulting in a recombinant retroviral vector. Conclusion The construction of eukaryotic expression vector of recombinant mouse CD1_D gene and B7-1 gene laid the foundation for further research.