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目的：研究维甲酸（ＲＡ）对人胃癌细胞株（ＳＧＣ７９０１）层粘素受体（ＬＮＲ）基因和原癌基因Ｃ－Ｈａ－ｒａｓ表达的影响。方法：应用原位杂交方法标记ＬＮＲ探针和Ｃ－Ｈａ－ｒａｓ探针，对人胃癌细胞（分为经ＲＡ处理及未经ＲＡ处理两组）中ＬＮＲ和Ｃ－Ｈａ－ｒａｓ基因的ｍＲＮＡ表达进行检测。结果：经ＲＡ处理的人胃癌细胞ＬＮＲ ｍＲＮＡ表达较未经ＲＡ处理者显著降低（Ｐ＜０．０１～０．００１）；Ｃ－Ｈａ－ｒａｓ转录水平亦显著降低（Ｐ＜０．０１～０．００１）。结论：ＲＡ可显著抑制人胃癌细胞ＬＮＲ和Ｃ－Ｈａ－ｒａｓ基因的表达。
Objective: To study the effect of retinoic acid (RA) on the expression of laminin receptor (LNR) gene and proto-oncogene C-Ha-ras in human gastric cancer cell line (SGC7901). METHODS: LNR and C-Ha-ras probes were used to label LNR and C-Ha-ras probes by in situ hybridization. The mRNA expression of LNR and C-Ha-ras genes in human gastric cancer cells (divided into RA-treated and non-RA-treated groups) was analyzed. Conduct the test. RESULTS: The expression of LNR mRNA in human gastric cancer cells treated with RA was significantly lower than that in those without RA treatment (P<0.01-0.001); the transcription level of C-Ha-ras was also significantly decreased (P<0.01 to 0). .001). Conclusion: RA can significantly inhibit the expression of LNR and C-Ha-ras genes in human gastric cancer cells.