【摘 要】
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A rapid,ultrasensitive and convenient fluorescence measurement technology based on the enhancement of the fluorescence intensity resulting from the interaction
【机 构】
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School of Chemistry and Chemical Engineering
论文部分内容阅读
A rapid,ultrasensitive and convenient fluorescence measurement technology based on the enhancement of the fluorescence intensity resulting from the interaction of functionalized CdSe/CdS quantum dots(QDs)with bovine serum albumin(BSA)was proposed.The citrate-stabilized CdSe/CdS(QDs)were synthesized by using Se powder and Na2S as precursors instead of any pyrophoric organometallic precursors.The modified CdSe/CdS QDs are brighter and more stable against photobleaching in comparison with organic fluorophores.At pH 7.0,the fluorescence signal of CdSe/CdS is enhanced by increasing the concentration of BSA in the range of 0.1-10 μg/mL,and the low detection limit is 0.06 μg/mL.A linear relationship between the enhanced fluorescence peak intensity(ΔF)and BSA concentration(c)is established using equation AF=50.7c+16.4(R=0.996 36).Results of determination for BSA in three synthetic samples are identical with the true values,and the recovery(98.9%-102.4%)and relative standard deviation(RSD,1.8%-2.5%)are satisfactory.
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