论文部分内容阅读
目的分析川乌炮制前后化学成分的变化,为深入阐明其炮制机理提供科学依据。方法采用高效液相色谱-质谱联用技术(HPLC-MS),Thermo SCIENTIFIC高效液相色谱仪,Agilent ExtendC18色谱柱,检测波长240 nm,流动相为乙腈和0.2%氨水,梯度洗脱,质谱在正离子模式下进行检测。结果生川乌中检测到99个成分,制川乌中检测到106个成分,炮制前后共有成分53个,炮制后新增成分53个,消失成分46个。川乌生制品中共鉴定成分64个。结论川乌炮制后有多种成分发生改变,本方法较高效液相色谱(HPLC)法检测6种生物碱含量能更全面地反映川乌炮制前后物质基础的变化。
Objective To analyze the changes of chemical composition before and after processing of Radix Aconiti Preparatum, and provide scientific basis for clarifying its processing mechanism. Methods High performance liquid chromatography-mass spectrometry (HPLC-MS), Thermo Scientific high performance liquid chromatography (HPLC) and Agilent Extend C18 column were used. The detection wavelength was 240 nm. The mobile phase consisted of acetonitrile and 0.2% Positive mode for testing. Results 99 samples were detected in Radix Chuanxiong, 106 components were detected in Radix Aconitum, 53 components were processed before and after processing, 53 were added after processing, and 46 were disappeared. Sichuan Wusheng products identified 64 ingredients. Conclusion There are many components of Radix salviae miltiorrhizae being changed after being processed. The determination of six alkaloids by HPLC method can more fully reflect the change of material base before and after processing.