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目的:研究肿瘤坏死因子(TNF)-α对角质形成细胞糖皮质激素受体(GR)表达的影响。方法:采用反转录实时定量PCR和Western blotting观察经TNF-α作用后,培养的人角质形成细胞株HaCaT细胞的GRα、GRβ mRNA和蛋白质的表达情况。结果:TNF-α对HaCaT细胞GRα的表达无明显影响,但可上调HaCaT细胞GRβ的表达,并且呈剂量和时间依赖。结论:TNF-α可增加GRβ的表达,可能是炎性细胞因子参与诱发糖皮质激素皮肤耐受现象的内在机制之一。
Objective: To investigate the effect of tumor necrosis factor (TNF) -α on the expression of glucocorticoid receptor (GR) in keratinocytes. Methods: Reverse transcriptional real-time PCR and Western blotting were used to observe the expression of GRα and GRβ mRNA and protein in cultured human keratinocytes HaCaT cells treated with TNF-α. Results: TNF-αhad no effect on the expression of GRα in HaCaT cells, but increased the expression of GRβ in HaCaT cells in a dose and time dependent manner. CONCLUSION: TNF-α can increase the expression of GRβ, which may be one of the intrinsic mechanisms involved in the induction of glucocorticoid skin tolerance by inflammatory cytokines.