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目的探讨白藜芦醇(resveratrol)衍生物白皮杉醇(piceatannol)和乙酰化白藜芦醇(acetylated resveratrol)对小鼠辐射损伤的防护作用。方法将昆明小鼠随机分为8组:正常对照组、照射对照组、白皮杉醇低、中、高剂量(50、100、200 mg/kg)组和乙酰化白藜芦醇低、中、高剂量(50、100、200 mg/kg)组,每组6只。小鼠经5.5 Gy 60Coγ射线一次性全身照射前2 h灌胃给药,对照组均灌服含2%DMSO的生理盐水溶液。照射后24 h,观察小鼠脾脏和胸腺系数、外周血淋巴细胞数量和百分率、血浆超氧化物歧化酶(superoxide dismutase,SOD)活力及骨髓嗜多染红细胞微核率的变化。结果与正常对照组相比,照射对照组小鼠脾脏和胸腺系数、外周血淋巴细胞数量和百分率及血浆SOD活力均显著降低(P<0.05或P<0.01),骨髓嗜多染红细胞微核率显著升高(P<0.01)。与照射对照组相比,白皮杉醇和乙酰化白藜芦醇各剂量组脾脏系数均显著增高(P<0.05或P<0.01),但胸腺系数差异无统计学意义(P>0.05);乙酰化白藜芦醇中、高剂量组外周血淋巴细胞数量和淋巴细胞百分率均显著升高(P<0.05或P<0.01);白皮杉醇中、高剂量组以及乙酰化白藜芦醇各剂量组血浆SOD活力均显著升高(P<0.05或P<0.01);白皮杉醇及乙酰化白藜芦醇各剂量组骨髓嗜多染红细胞微核率均显著降低(P<0.05或P<0.01)。结论白藜芦醇衍生物白皮杉醇和乙酰化白藜芦醇均对小鼠具有一定的辐射损伤防护作用,其作用机制与提高机体免疫能力、清除活性氧(reactive oxygen species,ROS)、保护造血组织免于辐射诱发的损伤有关。
Objective To investigate the protective effects of resveratrol derivative (piceatannol) and acetylated resveratrol (IR) against radiation injury in mice. Methods Kunming mice were randomly divided into 8 groups: normal control group, irradiation control group, low, medium and high dose of 50, 200 mg / kg and low, middle and high dose of acetated resveratrol , High dose (50,100,200 mg / kg) group, 6 rats in each group. The mice were given gavage 2 h before 5.5 Gy 60Coγ-ray whole body irradiation, and the control group were given physiological saline solution containing 2% DMSO. At 24 h after irradiation, the spleen and thymus coefficient, the number and percentage of peripheral blood lymphocytes, the activity of plasma superoxide dismutase (SOD) and the micronucleus rate of bone marrow polychromatic erythrocytes were observed. Results Compared with the normal control group, the spleen and thymus coefficient, the number and percentage of peripheral blood lymphocytes and the activity of plasma SOD in irradiated control group were significantly decreased (P <0.05 or P <0.01). The micronucleus rate of bone marrow polychromatic erythrocytes Significantly increased (P <0.01). Compared with irradiation control group, the spleen coefficient of each dose group of PES and acetylated resveratrol were significantly increased (P <0.05 or P <0.01), but the difference of thymus coefficient was not statistically significant (P> 0.05) The levels of peripheral blood lymphocytes and the percentage of lymphocytes in resveratrol group were significantly increased (P <0.05 or P <0.01), while those in high-dose and medium-dose resveratrol groups (P <0.05 or P <0.01). The micronucleus rate of bone marrow polychromatic erythrocytes in each dose group of PES and acetylated resveratrol were significantly lower (P <0.05 or P <0.01). Conclusions Both resveratrol derivatives and acetylated resveratrol can protect mice against radiation injury. Its mechanism of action is to improve immunity, eliminate reactive oxygen species (ROS) and protect mice Hematopoietic tissue is free of radiation-induced damage.