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对５８例癌性腹水及２５例癌性胸水用ＭＴＴ法进行抗癌药物敏感试验。用临床常规方法收集胸腹水，并用肝素抗凝（１０ｕ／ｍｌ）；用不连续密度梯度离心去除渗出液中红白细胞，制成肿瘤单细胞悬液，进行药敏测试。同时观察到卵巢癌患者的实体瘤与腹水之间的肿瘤细胞存活率无统计学意义的不同，相关性较好。应用药敏结果进行胸腹腔给药治疗，效果较好，６０％～７０％患者胸腹水近期得到控制，这对于延长患者生命是有益的。
Forty-eight cases of cancerous ascites and 25 cases of cancerous pleural effusion were tested for anticancer drug sensitivity by MTT assay. Pleural effusion and ascitic fluid were collected by clinical routine methods and anticoagulated with heparin (10 u/ml); red blood cells in exudates were removed by discontinuous density gradient centrifugation to prepare single cell suspensions for drug susceptibility testing. At the same time, it was observed that the survival rate of tumor cells between solid tumors and ascites in patients with ovarian cancer was not statistically different, and the correlation was good. The use of susceptibility results in the treatment of thoracic cavity and abdominal cavity is effective, and 60% to 70% of the patients’ pleural effusion and ascites fluid are recently controlled, which is beneficial for extending the life of patients.