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目的:7-二氟甲氧基-5,4’-二甲氧基金雀异黄素(7-difluoromethoxy-5,4’-dimethoxygenistein,dFMG)对溶血性磷脂酰胆碱(lysophosphatidylcholine,LPC)诱导人脐静脉内皮细胞(HUVE-12)损伤模型的保护作用,探讨其是否通过抑制CD40/CD40L相互作用E-选择素的释放相关。方法:体外培养人脐静脉内皮细胞(HUVE-12)。Annexin V/PI染色流式细胞术(FCM)分析细胞凋亡率,用流式细胞仪检测CD40/CD40L的表达,酶联免疫吸附法检测E-选择素的释放。结果:LPC(10μmol/L)处理HUVE-12(6h、12h、24h和48h),24h的细胞凋亡率为(30.98%±2,04%)(P<0.05)。DFMG预孵育降低LPC诱导HUVE-12的细胞凋亡率,CD40/CD40L的表达降低及E-选择素的释放减少(P<0.05)。结论:DFMG拮抗LPC诱导HUVE-12细胞凋亡作用与减少CD40的表达及E-选择素的释放有关。
OBJECTIVE: Induction of hemolytic phosphatidylcholine (LPC) by 7-difluoromethoxy-5,4’-dimethoxygenistein (dFMG) To investigate the protective effect of HUVE-12 on the injury model of human umbilical vein endothelial cells (HUVECs) and to explore whether it is related to the release of E-selectin by inhibiting the interaction of CD40 / CD40L. Methods: Human umbilical vein endothelial cells (HUVE-12) were cultured in vitro. Annexin V / PI staining flow cytometry (FCM) analysis of apoptosis rate, flow cytometry CD40 / CD40L expression, enzyme-linked immunosorbent assay E-selectin release. Results: The apoptotic rates of HUVE-12 treated with LPC (10μmol / L) for 6 h, 12 h, 24 h and 48 h were (30.98% ± 2.04%) (P <0.05). Preincubation with DFMG decreased the apoptosis rate of HUVE-12 induced by LPC, decreased the expression of CD40 / CD40L and decreased the release of E-selectin (P <0.05). CONCLUSION: DFMG antagonism of LPC-induced HUVE-12 cell apoptosis is related to the decrease of CD40 expression and E-selectin release.