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本研究采用裂区试验设计,主区设置了3种覆膜方式:露地平播(即常规播种方法,无覆膜)、半覆膜平作(即常规播种方法,覆膜占小区面积一半)、全膜垄上穴播(即起垄后小区全覆膜,垄上播种),副区设置了2个丛枝菌根(AM)真菌接种水平:接种AM真菌(AM)和不接种对照(CK),研究了大田条件3种覆膜方式下接种AM真菌对半干旱区春播玉米根际土壤养分、有机碳含量及AM真菌特性(侵染率、根外菌丝密度与土壤球囊霉素)的影响。结果表明:3种覆膜方式下,与不接种对照相比,接种AM真菌显著提高了根系侵染率、根外菌丝密度、土壤中球囊霉素和有机碳含量、植株干重、碳氮比和土壤含水量,同时显著促进了土壤养分吸收(个别例外),其中土壤根外菌丝密度、易提取球囊霉素、有机碳、速效磷和速效钾含量、碳氮比随着覆膜方式由无覆膜-半覆膜-全覆膜的变化呈降低趋势,而植株干重、土壤中总球囊霉素、全氮和含水量随着覆膜方式由无覆膜-半覆膜-全覆膜的变化呈升高的趋势。全覆膜结合接种AM真菌在促进西北半干旱地区田间作物生长、提高土壤含水量、以及改善菌根侵染率、菌丝密度与土壤中球囊霉素含量的作用最大,但降低了土壤养分,后期还可能需要通过合理施肥措施加以维持土壤肥力水平。相关分析表明,土壤根外菌丝密度和球囊霉素含量与土壤矿质养分和水分存在一定程度的协同效应,接种AM真菌有助于根际土壤养分转化,促进植物生长。
In this study, split-plot experiment design was adopted. Three kinds of coating methods were set up in the main area: open-field sowing (conventional sowing method without sowing), semi-sowing and sowing (ie conventional sowing method, Two AM fungi inoculation levels were set up in the subzone: AM fungi (AM) and non-inoculation control (CK) The effects of AM fungi on rhizosphere soil nutrient, organic carbon content and AM fungi (infection rate, mycorrhizal hyphal density and soil gemcitabine) of spring sowing maize in semi-arid area were studied under field conditions. influences. The results showed that inoculation of AM fungi significantly increased root infection rate, extracellular mycorrhizal density, soil contents of ascomycin and organic carbon, plant dry weight, carbon content Nitrogen ratio and soil water content, and significantly promoted soil nutrient absorption (with some exceptions). Among them, the mycorrhizal density outside soil, the content of extracellular glycoconjugate, organic carbon, available phosphorus and available potassium, C / The change of membrane-free membrane-half-membrane-full-membrane showed a decreasing trend, while the dry weight of plant, the total amount of glomcnin, total nitrogen and water content in the soil were increased by the uncoated- Membrane - the changes of the whole film showed an upward trend. The combination of AMF and AMF could promote crop growth, increase soil water content and improve mycorrhizal infection rate in the semi-arid area of Northwest China. The effect of mycorrhizal density on the mycorrhizal density was the greatest, but the soil nutrients Later, it may also be necessary to maintain soil fertility through rational fertilization measures. Correlation analysis showed that the mycorrhizal fungi inoculated with AM fungi could promote the transformation of rhizosphere soil and promote the growth of plants.