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从众多水稻品种及单植株中评价筛选少数具有化感特性的材料，是选育水稻化感新品种取得突破的关键．具有化感特性的水稻品种及单植株通过产生和释放特定次生物质到环境中而显示化感效应．这样，利用高效液相色谱技术，以这些特征次生物质为标记可以评价水稻材料的化感潜力．用这一评价方法仅１年就评价了３０００个水稻品种和部分杂交子系的Ｆ３和Ｆ４植株的化感潜力，而用传统的田间评价方法则需要１０余年．而且这一评价方法还能指导和监控水稻化感品种选育过程．经液相色谱－质谱和核磁共振等技术分离和结构鉴定，证明水稻的化感物质是糖甙间烃基苯二酚、黄酮和羟基肟酸，而不是普遍认为的酚酸和脂肪酸，但这些糖甙分子释放到环境中在微生物和酸性媒介的作用下迅速降解成糖、酚酸和脂肪酸等小分子．
Evaluating and screening a small number of materials with allelopathic features from a large number of rice varieties and single plants is the key to breakthrough in breeding a new rice allelopathy variety. Allelopathic rice varieties and single plants show an allelopathic effect by generating and releasing specific secondary substances into the environment. In this way, the potential allelopathic potential of rice materials can be evaluated using high-performance liquid chromatography techniques with these characteristic subsites as markers. Using this evaluation method, the allelopathic potential of F3 and F4 plants of 3000 rice varieties and some hybrid lines was evaluated in just one year, whereas traditional field evaluation methods took more than 10 years. And this evaluation method can also guide and monitor the breeding process of rice allelopathy. Isolation and structural identification by liquid chromatography-mass spectrometry and nuclear magnetic resonance techniques demonstrated that the allelochemicals of rice are glycosidic hydroquinones, flavonoids and hydroxamic acids rather than the commonly recognized phenolic acids and fatty acids, but these sugars Glycosides released to the environment in the environment under the action of microorganisms and acidic medium quickly degraded into sugar, phenolic acids and fatty acids and other small molecules.