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以菜花为碳源,通过一步水热法合成了绿色荧光碳量子点,通过荧光光谱、紫外-可见吸收光谱、红外光谱对其发光特性进行了研究,测定了其荧光寿命及量子产率。研究发现,塞克硝唑对该碳量子点荧光具有明显的猝灭作用,在pH 7.10的Tris-HCl缓冲溶液中,碳量子点的荧光猝灭强度与塞克硝唑浓度在7.0×10~(-7)~3.0×10~(-5)mol/L范围内呈线性,相关系数为0.9991,方法检出限为1.9×10~(-7)mol/L。该方法用于样品中塞克硝唑的测定,回收率为98.8%~101.5%。通过荧光寿命和吸收光谱的变化以及温度对猝灭常数的影响,确定该碳量子点与塞克硝唑的猝灭机理为动态猝灭。
Using cauliflower as carbon source, green fluorescent carbon quantum dots were synthesized by one-step hydrothermal method. Their luminescence properties were studied by fluorescence spectroscopy, UV-Vis absorption spectrum and infrared spectroscopy. Their fluorescence lifetime and quantum yield were measured. The study found that metforidazole has a significant quenching effect on the fluorescence of the carbon quantum dots. In the pH 7.10 Tris-HCl buffer solution, the fluorescence quenching intensity of the carbon quantum dots is about 7.0 × 10 ~ (-7) -3.0 × 10 -5 mol / L, the correlation coefficient was 0.9991, and the detection limit was 1.9 × 10 -7 mol / L. The method was applied to the determination of Secnidazole in the samples with recovery of 98.8% ~ 101.5%. Through the change of fluorescence lifetime and absorption spectrum and the influence of temperature on the quenching constant, the quenching mechanism of the carbon quantum dots and Secnidazole is determined to be dynamic quenching.