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目的在中国汉族人群中探讨TNFA基因启动子区单核苷酸多态性是否与HBV感染结局相关联。方法以148例HBV自限性感染者和207例慢性乙肝患者为研究对象,应用聚合酶链反应-限制性片段长度多态性和序列特异性引物-PCR方法对TNFA基因启动子区5个位点,-238G/A、-308G/A、-857C/T、-863C/A和-1031T/C进行基因型分型,用EPI和EH等统计学软件分析各位点等位基因、基因型、单倍型频率及其组间差异。结果TNFA基因-238位GG基因型在慢性肝炎组的频率显著高于自限性感染组(P=0.02),-857TT基因型的频率在慢性肝炎组显著低于自限性感染组(P=0.02)。TNFA基因-238/-308/-857/-863/-1031组成的单倍型GGCCT的频率在慢性肝炎组显著低于自限性感染组(P=0.03),单倍型GGCAT与GGTAT在慢性肝炎组的频率显著高于自限性感染组(P=0.0001,P=0.004)。结论TNFA基因启动子区多态性与HBV感染结局显著关联。
Objective To investigate whether SNFA gene promoter SNPs are associated with HBV infection in Chinese Han population. Methods A total of 148 patients with HBV self-limited infection and 207 patients with chronic hepatitis B were selected as study subjects. Five loci in the promoter region of TNFA gene, including polymerase chain reaction-restriction fragment length polymorphism and sequence-specific primer-PCR, Point, -238G / A, -308G / A, -857C / T, -863C / A and -1031T / C were genotyped and analyzed by EPI and EH statistical software such as allele, genotype, Haplotype frequencies and their differences between groups. Results The frequency of GFA genotype at position-238 of TNFA gene in chronic hepatitis group was significantly higher than that in self-limited infection group (P = 0.02). The frequency of - 857TT genotype was significantly lower in chronic hepatitis group than in self-limited infection group (P = 0.02). The frequency of haplotype GGCCT consisting of TNFA gene -238 / -308 / -857 / -863 / -1031 was significantly lower in chronic hepatitis group than in self-limited infection group (P = 0.03). The frequencies of haplotype GGCAT and GGTAT in chronic The frequency of hepatitis group was significantly higher than that of self-limited infection group (P = 0.0001, P = 0.004). Conclusion TNFA gene promoter polymorphism is significantly associated with HBV infection.