目的 :建立测定异维A酸血药浓度的方法。方法 :在避光条件下 ,血浆标本加磷酸盐缓冲液后用乙醚提取 ,吹干后以二氯甲烷 -冰醋酸 (99 5∶0 5 )为流动相 ,经过YWGSi6 0柱分离后在紫外 36 5nm处检测。结果 :血药浓度标准曲线在 10～ 5 0 0ng·mL-1,r=0 9999,最低检测限为 10ng mL-1,高、中、低 3种浓度平均回收率为 81 6 2 %～ 8 8 99%。日内和日间平均RSD小于 0 6 1%和 8 2 0 %。结论 :本法可为异维A酸的药动学研究提供检测方法 Objective: To establish a method for determination of isotretinoin concentration. Methods: Under the dark conditions, the plasma samples were extracted with ether, washed with ether, dried with dichloromethane - glacial acetic acid (99 5:0 5) as mobile phase, separated on a YWGSi6 0 column and incubated in UV 36 5nm at the detection. Results: The standard curve of plasma concentration was 10 ~ 500 ng · mL-1, r = 0 9999, and the lowest detection limit was 10 ng mL-1. The average recoveries of high, medium and low concentrations were 81 6 2% -8 8 99%. The average daily and intraday RSDs were less than 0.661 and 82.0%. Conclusion: This method can provide a test method for the pharmacokinetics of isotretinoin