Single crystal Ni-based superalloys are the typical structural materials for high-pressure turbine blades, and their microstructure is critical in determining their mechanical properties. The withdrawal rate is a key parameter affecting the microstructure during the single crystal growth process. In the present work the effect of the withdrawal rate on the microstructure of a third-generation single crystal superalloy containing 6.8 wt% Re has been investigated, and the creep resistance of the alloy determined. The results showed that increased withdrawal rate refined the dendritic structure, reduced dendritic arm spacing, promoted the growth of secondary tertiary dendrites and decreased solidification segregation with a reduced size of γ phase. The porosity density of the as-cast alloy first decreased and then increased with the withdrawal rate, while the minimum porosity densi-tyoccurred when the alloy was under the solidification condition of withdrawal rate of 4.5 mm/min. The maximum creep rupture life of 326.4 h of the heat-treated alloys under the test condition of 1100 C/140 MPa also appeared at the alloys under the withdrawal rates of 4.5 mm/min. It is believed that the minimum porosity density and reduced size of the γ phase may be the main reasons for the enhanced creep rupture life of the alloys with withdrawal rates of 4.5 mm/min. This investigation provides theoretical support and a practical basis for the development of third-generation single crystal superalloys.