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为了解安徽省山羊蓝氏贾第鞭毛虫的流行及基因型,本研究对安徽省六安、阜阳、宿州、池州、芜湖、滁州和亳州的506份山羊粪样采用卢戈氏碘液染色镜检法进行鉴定,对鉴定出的蓝氏贾第鞭毛虫阳性粪样,进行基于磷酸丙糖异构酶(TPI)和谷氨酸脱氢酶(GDH)基因的巢式PCR扩增,并对阳性产物进行测序,序列用Clustal X 1.81和Mega 5.05软件进行比对分析。镜检结果显示,共检出32份蓝氏贾第鞭毛虫阳性粪样,总感染率为6.3%。巢式PCR扩增结果显示,32份阳性粪样中,TPI基因扩增阳性23份,GDH基因扩增阳性16份,产物大小分别为530 bp和450 bp。序列分析表明,分离的虫株均为反刍动物特有的聚集体E,但基因亚型存在地区性分布差异,未发现具有人兽共患潜力的A型或B型。
In order to understand the prevalence and genotypes of Giardia lamblia in Anhui Province, 506 Goat fecal specimens from Lu’an, Fuyang, Suzhou, Chizhou, Wuhu, Chuzhou and Bozhou of Anhui Province were collected using Lugol’s iodine staining microscope The method was used to identify the positive samples of the positive samples of Giardia lamblia which were identified by nested PCR based on the gene of triosephosphate isomerase (TPI) and glutamate dehydrogenase (GDH) The positive product was sequenced and the sequences were aligned using Clustal X 1.81 and Mega 5.05 software. Microscopic examination showed that 32 positive samples of Giardia lamblia positive were detected, with a total infection rate of 6.3%. The results of nested PCR showed that in 32 positive fecal specimens, 23 positive for TPI gene amplification and 16 positive for GDH gene amplification were obtained, with product sizes of 530 bp and 450 bp, respectively. Sequence analysis showed that all the isolates were ruminants’ unique aggregates E, but there was a regional distribution of genotypes. No type A or B with zoonotic potential was found.