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目的：研究三七总皂苷（ｓａｐｏｎｉｎｓｏｆＰａｎａｘｎｏｔｏｇｉｎｓｅｎｇ，ＰＮＳ）对不同状态下脑细胞内游离钙离子浓度（［Ｃａ２＋］ｉ）的影响。方法：采用Ｆｕｒａ２作为荧光指示剂，测定新生大鼠脑细胞中荧光强度的变化。结果：ＰＮＳ１００，４００ｍｇ·Ｌ－１对高钾（５０ｍｍｏｌ·Ｌ－１）、谷氨酸（１００μｍｏｌ·Ｌ－１）诱导的以及缺氧引起的［Ｃａ２＋］ｉ增高均有明显的抑制作用。结论：ＰＮＳ是钙离子拮抗剂
OBJECTIVE: To study the effects of saponins of Panaxnotoginseng (PNS) on intracellular free calcium concentration ([Ca2+]i) in brain cells under different conditions. Methods: Fura-2 was used as a fluorescent indicator to measure the changes of fluorescence intensity in neonatal rat brain cells. RESULTS: PNS 100,400 mg·L-1 significantly inhibited the increase of [Ca2+]i induced by high potassium (50 mmol·L-1), glutamic acid (100 μmol·L-1) and hypoxia. Conclusion: PNS is a calcium antagonist