Altomare DF、Guanti G、Hoch J等为探讨R0切除的结直肠癌患者术前骨髓中的非结肠型肿瘤干细胞是否是癌细胞并且是否会影响肿瘤肝脏转移的发生率,从5个研究中心前瞻性纳入结直肠癌患者,于开腹手术中抽取骨髓并由同一实验室进行分析。依据国际抗癌联合会制订的标准判断非结肠型肿瘤干细胞,对每例患者约3×106个骨髓细胞进行角蛋白20单克隆抗 Altomare DF, Guanti G, Hoch J et al. To investigate whether preoperative colorectal cancer patients with R0-resected colorectal cancer are non-colorectal cancer stem cells and whether they affect the incidence of liver metastases, Patients with colorectal cancer were enrolled in the open surgery and were analyzed by the same laboratory. Based on the criteria established by the International Union Against Cancer, non-colon cancer stem cells were determined and about 3 × 10 6 bone marrow cells per patient were subjected to monoclonal keratin 20