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目的:建立RP-HPLC同时测定金银花药材中绿原酸、金丝桃苷和木犀草苷的含量的方法。方法:HiQ silC18(4.6 mm×250 mm,5μm)色谱柱;流动相A为乙腈,B为磷酸-水(99.5∶0.5);梯度洗脱程序:0~10 min,13%A;10~12 min,13%~20%A;12~24 min,20%~21%A;24~32 min,21%~23%A;检测波长355 nm;流速1.0 mL/min;柱温30℃。测定了6个产地金银花药材中3种成分的含量。结果:绿原酸、金丝桃苷和木犀草苷分别在2.265~9.060μg(r=0.9996),0.049~0.194μg(r=0.9997),0.079~0.315μg(r=0.9997)范围内线性关系良好。不同产地金银花药材中3种成分的含量差异较大。结论:本方法简便快速、准确度高、重现性好,可作为金银花药材的质量控制方法。
Objective: To establish a method for simultaneous determination of chlorogenic acid, hyperoside and luteolin in honeysuckle by RP-HPLC. Methods: The chromatographic column was HiQ silC18 (4.6 mm × 250 mm, 5 μm). The mobile phase A was acetonitrile and B was phosphoric acid-water (99.5: 0.5). The gradient elution program was 0-10 min, 13% min, 13% -20% A, 12-24 min, 20-21% A, 24-32 min, 21-23% A, detection wavelength 355 nm, flow rate 1.0 mL / min and column temperature 30 ℃. The contents of three components in six honeysuckle medicinal materials were determined. Results: There was a good linear relationship between chlorogenic acid, hyperoside and luteolin in the range of 2.265-9.060μg (r = 0.9996), 0.049-0.1994μg (r = 0.9997), 0.079-0.315μg (r = 0.9997) . Different origin honeysuckle herbs in the content of three different components. Conclusion: The method is simple, rapid, accurate and reproducible. It can be used as a quality control method for honeysuckle.