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目的:探讨肺癌患者干扰素-γ(IFN-γ)和肿瘤坏死因子-α(TNF-α)检测的临床意义。方法:选择我院2009-02/2010-04收治的肺癌患者84例作为实验组,77例健康患者作为对照组,分别静脉采血后,采用双抗体夹心ELISA法检测并分析IFN-γ和TNF-α的生物活性,并对两组患者以及实验组患者化疗后半年内IFN-γ和TNF-α的水平进行比较研究。结果:与对照组正常人群比较,实验组IFN-γ水平明显降低,而TNF-α水平明显提高,差异均具有统计学意义(P<0.05)。与此同时,化疗后半年内,与未复发组相比,复发组IFN-γ水平明显提高,而TNF-α水平明显降低,差异均具有统计学意义(P<0.05)。结论:更加深入地探讨肺癌患者的免疫损伤机制将有助于更好地指导治疗,并且具有重要的临床意义。
Objective: To investigate the clinical significance of detecting interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) in patients with lung cancer. Methods: Eighty-four patients with lung cancer who were treated in our hospital from February 2009 to April 2010 were selected as the experimental group and 77 healthy patients as the control group. After venous blood was taken from the hospital, the double antibody sandwich ELISA was used to detect and analyze the expression of IFN-γ and TNF- α biological activity, and the two groups of patients and experimental patients within six months after chemotherapy, IFN-γ and TNF-α levels were compared. Results: Compared with the normal control group, the level of IFN-γ in the experimental group was significantly decreased, while the level of TNF-α was significantly increased. The difference was statistically significant (P <0.05). In the meantime, the level of IFN-γ in the recurrent group was significantly higher than that in the non-recurrent group, but the level of TNF-α was significantly lower in the first half of chemotherapy (P <0.05). CONCLUSIONS: A more in-depth study on the mechanism of immune damage in patients with lung cancer will help to guide the treatment and has important clinical significance.