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研究植物源性发酵乳杆菌对免疫系统生理活性、抗高血糖和促进肠蠕动的影响。β-内啡肽对免疫系统有重要作用,阿片-促黑素细胞皮质素原(POMC)是β-内啡肽的前体物质,为了探讨发酵乳杆菌对免疫功能的影响,检测小鼠下丘脑和脑垂体中POMC的基因表达水平。通过喂食发酵乳杆菌时间和浓度的不同及测量β-内啡肽表达水平的方式,来分析血液中β-内啡肽的变化情况。此外,测定了三种小鼠模型的血糖变化,即常规的WT小鼠模型、D-葡萄糖诱导的急性高血糖模型和链唑霉素(STZ)诱导的糖尿病模型,以证实发酵乳杆菌的降血糖效果,并对降血糖效果进行了验证。通过检测血糖随喂食发酵乳杆菌浓度和时间而变化的情况,已证实给药浓度和时间对血糖浓度有很好的影响。另外,为证实发酵乳杆菌对肠蠕动的影响测定了胃肠通过时间,证明发酵乳杆菌对肠蠕动有极好的促进作用。通过此项研究,证实发酵乳杆菌对提高人体免疫系统的生理活性、抗高血糖和促进小肠蠕动有极佳的效果。
To study the effects of Lactobacillus plantarum on immune system physiological activity, anti-hyperglycemia and intestinal motility. β-endorphin plays an important role in the immune system. Opioid-melanocyte pro-cortin (POMC) is a precursor of β-endorphin. To investigate the effect of Lactobacillus fermentum on immune function, POMC gene expression levels in thalamus and pituitary gland. The changes of β-endorphin in blood were analyzed by the difference of the time and concentration of feeding Lactobacillus fermentum and the way of measuring the expression of β-endorphin. In addition, changes in blood glucose were measured in three mouse models, namely the conventional WT mouse model, the D-glucose-induced acute hyperglycemia model, and the streptozotocin (STZ) -induced diabetic model to confirm the decrease of the Lactobacillus fermentum Blood sugar effect, and hypoglycemic effect was verified. By detecting the change of blood glucose with the concentration and time of feeding Lactobacillus fermentum, it has been confirmed that the administration concentration and time have a good effect on the blood glucose level. In addition, in order to confirm the influence of fermented lactobacillus on intestinal peristalsis, the passage time of gastrointestinal tract was determined, which proved that Lactobacillus fermentum had an excellent promoting effect on peristalsis. Through this study, it was confirmed that Lactobacillus fermentum has an excellent effect on improving the physiological activity of the human immune system, resisting high blood sugar and promoting small intestine peristalsis.