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目的 :观察羊膜对在其上皮面培养的兔角膜上皮细胞增生的影响。方法 :将兔角膜上皮细胞原代培养后 ,接种于羊膜上皮面 ,并用MTT自动比色法分别于接种后 1、3、5、7天检测羊膜对兔角膜上皮细胞增生的影响。结果 :接种后 1 ,3,5天 ,羊膜对兔角膜上皮细胞有明显的促增生作用 (P <0 0 5)。结论 :羊膜有促进兔角膜上皮细胞增生的作用 ,可用做角膜表面结构重建的理想材料。
Objective: To observe the effect of amniotic membrane on the proliferation of rabbit corneal epithelial cells cultured on its epithelial surface. Methods: Primary cultured rabbit corneal epithelial cells were inoculated on the epithelium of amniotic membrane. The effects of amniotic membrane on the proliferation of rabbit corneal epithelial cells were detected by MTT assay at 1, 3, 5 and 7 days after inoculation. Results: On day 1, day 3 and post-inoculation, amniotic membrane had a significant promoting effect on rabbit corneal epithelial cells (P <0.05). Conclusion: Amniotic membrane can promote the proliferation of rabbit corneal epithelial cells, which can be used as an ideal material for reconstruction of corneal surface structure.