论文部分内容阅读
目的：在大鼠心肌细胞水平研究缺氧再给氧时克罗卡林对心肌细胞内丙二醛（ＭＤＡ）含量、超氧化物歧化酶（ＳＯＤ）活性及心肌细胞膜脂质流动性的影响，探讨克罗卡林抗脂质过氧化作用及其可能机制。方法：实验用培养的Ｓｐｒｅｇｕｅ－Ｄａｗｌｅｙ（ＳＤ）乳鼠原代心肌细胞，分对照组、缺氧再给氧组及缺氧再给氧＋克罗卡林组（又分３个浓度组，即１×１０－４ｍｏｌ／Ｌ组、１×１０－５ｍｏｌ／Ｌ组，１×１０－６ｍｏｌ／Ｌ组）。每组观察５个培养瓶，分别观察以下指标：心肌细胞内ＭＤＡ含量（荧光微量法）；心肌细胞内ＳＯＤ活性（光化学扩增法）；心肌细胞膜脂质流动性（ＤＰＨ法），以各向异性ｒ值表示膜脂质流动性的大小。数据以ｘ±ｓ表示，组间差异显著性检测用方差分析法。Ｐ＜０．０５为差别有显著性。结果：缺氧再给氧组心肌细胞内ＭＤＡ含量增加，ＳＯＤ活性降低，心肌细胞膜脂质流动性下降（与对照组比较，Ｐ均＜０．０１）；缺氧再给氧＋克罗卡林组上述改变减轻，其中１×１０－５ｍｏｌ／Ｌ组、１×１０－４ｍｏｌ／Ｌ组上述指标与缺氧再给氧组及１×１０－６ｍｏｌ／Ｌ组比较，Ｐ均＜０．０１，呈良好的量效关系。结论：缺氧再给氧时细胞内脂质过氧化作用增强；克罗卡林明显减轻缺氧?
OBJECTIVE: To study the effects of crokalin on the content of malondialdehyde (MDA), the activity of superoxide dismutase (SOD) and the lipid permeability of myocardial cells in neonatal rat cardiomyocytes at hypoxia- To investigate the anti-lipid peroxidation of cocaine and its possible mechanism. Methods: Primary cultured cardiomyocytes of Sprague-Dawley (SD) rats were divided into control group, hypoxia reoxygenation group and hypoxia reoxygenation + croneir group (divided into three concentration groups, namely 1 × 10-4mol / L group, 1 × 10-5mol / L group, 1 × 10-6mol / L group). Five culture flasks were observed in each group, and the following indexes were observed: MDA content in myocardial cells (fluorescence microdialysis method); SOD activity in cardiomyocytes (photochemical amplification method); lipid membrane fluidity in cardiomyocytes membrane (DPH method) The opposite sex r value indicates the size of the membrane lipid fluidity. Data to x ± s said significant differences between groups using analysis of variance. P <0.05 for the difference was significant. Results: Compared with the control group, the MDA content and the activity of SOD in the hypoxia and reoxygenation groups were decreased (P <0.01, P <0.01) The above changes were alleviated in the groups of 1 × 10-5mol / L and 1 × 10-4mol / L, P <0.01 compared with those in hypoxia-reoxygenation group and 1 × 10-6mol / L group, A good dose-effect relationship. CONCLUSION: Intracellular lipid peroxidation is enhanced by hypoxia-reoxygenation. Chloramphenicol significantly reduces hypoxia.