论文部分内容阅读
为了探讨烟粉虱取食对辣椒体内营养物质和抗性物质的影响,选择抗虫辣椒品种(‘新一代三鹰椒’、‘新苏椒五号’)和感虫品种(‘苏椒13号’、‘苏椒15号’)各2个,每株辣椒分别接烟粉虱成虫0头(对照)、30头(低密度)、60头(中密度)和120头(高密度),取食24 h后测定辣椒叶片中叶绿素、可溶性糖、可溶性蛋白以及酚类、黄酮类化合物含量。结果发现,低密度的烟粉虱取食后,辣椒叶片叶绿素含量显著上升,随着虫口密度的提高,叶绿素含量有所下降,但仍高于对照;感虫辣椒品种的叶绿素含量增加幅度高于抗虫品种。低密度和高密度的烟粉虱取食后,辣椒叶片中可溶性糖含量出现明显下降趋势,抗虫品种的下降幅度大于感虫品种。低密度和中密度的烟粉虱取食后,辣椒叶片中可溶性蛋白含量显著下降,但高密度烟粉虱取食后辣椒叶片中可溶性蛋白含量明显上升;抗虫品种的变化幅度明显高于感虫品种。烟粉虱取食后,叶片中酚类化合物含量明显上升,感虫品种的上升幅度较抗虫品种上升幅度大;随着虫量的增加,辣椒叶片中酚类化合物含量呈明显上升趋势,但高密度烟粉虱取食后酚类化合物含量出现明显下降趋势。烟粉虱取食后,感虫辣椒品种‘苏椒15号’叶片中黄酮类化合物含量明显上升;其他3个品种在中等以下密度的烟粉虱取食时,黄酮类化合物与对照差异不显著,但在高密度烟粉虱取食时,黄酮类化合物明显高于对照。研究表明,烟粉虱取食可引起辣椒营养物质和抗性物质含量向着有利于提高抗虫性的方向改变,但不同虫口密度下不同辣椒品种的改变幅度不同。研究结果为进一步揭示寄主植物与烟粉虱之间的防御和反防御机制提供了基础。
In order to investigate the effects of Bemisia tabaci feeding on nutrients and resistant substances in pepper, we selected pest-resistant pepper varieties (’new generation Sanjiaojiao’ and ’Xinzaojiao 5’) and susceptible varieties (’Sujiao 13 No.2, No.2 and No.2 respectively. Each pepper was fed with 0 adult whitefly (control), 30 heads (low density), 60 heads (medium density) and 120 heads (high density) The contents of chlorophyll, soluble sugar, soluble protein, phenolic compounds and flavonoids in pepper leaves were determined 24 h after feeding. The results showed that chlorophyll content of pepper leaves increased significantly after low-density feeding on B. tabaci, while the content of chlorophyll decreased but the content of chlorophyll decreased, but the chlorophyll content of peppers and peppers increased more than Insect-resistant varieties. Low-density and high-density B. tabaci feeding, the soluble sugar content of pepper leaves showed a significant downward trend, insect-resistant varieties decreased more than susceptible varieties. After low and medium density B. tabaci feeding, the content of soluble protein in pepper leaves decreased significantly, but the content of soluble protein in pepper leaves increased significantly after feeding on high density B. tabaci. The variation range of resistant varieties was obviously higher than that of susceptible ones Insect species. After feeding on B. tabaci, the content of phenolic compounds in leaves increased significantly, and the increase rate of susceptible varieties was higher than that of insect-resistant varieties. With the increase of insect quantity, the content of phenolic compounds in pepper leaves increased obviously, The content of phenolic compounds in high-density whiteflies decreased significantly after feeding. After feeding on whiteflies, the content of flavonoids in the leaves of the sensitive peppers ’Sujiao 15’ increased significantly. The other three cultivars showed no significant difference in the flavonoids from the control when they were fed on medium-density Bemisia tabaci , But the flavonoids were obviously higher than the control when they were fed on high-density whiteflies. Studies have shown that B. tabaci feeding can cause pepper nutrients and resistant substances to increase the direction conducive to improving insect resistance, but different pest population density of different pepper varieties vary in magnitude. The results provide the basis for further revealing the defense and anti-defense mechanisms between host plants and B. tabaci.