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目的 通过靶肌肉 (腓肠肌 )注射不同剂量的甲钴胺 ,观察其对大鼠坐骨神经损伤的再生作用 ,为临床肌肉局部注射甲钴胺促进周围神经再生探讨较佳的用药剂量和方法。 方法选用健康Wistar大鼠 30只 ,左侧坐骨神经横断后即刻行外膜缝合 ,制备大鼠坐骨神经损伤模型。随机分为A、B、C三组 ,每组 1 0只 ,A组注射甲钴胺 30 0 μg kg,B组注射甲钴胺 1 0 0 μg kg ,C组注射同体积的等渗盐水 1ml,术后靶肌肉注射 1次 d。术后第 4周、第 8周每组分别提取 5只大鼠 ,以左小腿三头肌湿重、光镜和电镜坐骨神经形态学观察并图像分析作为检测指标 ,分析不同剂量甲钴胺对大鼠坐骨神经损伤的影响。 结果 术后 4周左小腿三头肌湿重 ,A组为 (1 .36 7± 0 .0 1 2 )g ,B组为 (1 .1 6 4± 0 .0 1 1 )g,C组为 (0 .95 0± 0 .0 0 9)g ,差异有显著性意义 (P <0 .0 5 ) ;术后 8周 ,A组为 (2 .2 0 5± 0 .0 1 5 )g ,B组为 (1 .6 1 1± 0 .0 1 3)g ,C组为 (1 .2 30± 0 .0 1 4 )g ,差异有显著性意义 (P <0 .0 5 )。术后 4周和 8周行光镜和电镜观察 ,图像分析坐骨神经髓鞘的横截面积、壁厚度 ,A组优于B组 ,B组优于C组。 结论 靶肌肉注射甲钴胺可以促进周围神经的再生 ,高剂量的甲钴胺可以更好地发挥其对损伤神经的营养诱导作用 ,值
Objective To observe the regenerative effect of mecobalamin on the sciatic nerve injury in rats by injecting different doses of mecobalamin into the target muscle (gastrocnemius muscle), and to explore the best dosage and method for clinical muscular injection of mecobalamin to promote peripheral nerve regeneration. Methods Thirty healthy Wistar rats were selected. The left sciatic nerve was transected and the outer sciatic nerve was sutured to prepare the sciatic nerve injury model in rats. A group was randomly divided into three groups A, B and C, 10 in each group. Group A received mecobalamin 30 0 μg kg, group B received mecobalamin 100 μg kg, group C injected isotonic saline 1 ml , After intramuscular injection of target 1 d. At the 4th and 8th week after operation, 5 rats were taken from each group. The left triceps wet weight, light microscope and electron microscope sciatic nerve morphological observation and image analysis were taken as the detection indexes. Effect of rat sciatic nerve injury. Results Left thigh triceps wet weight at 4 weeks after operation was (1.36 7 ± 0. 01 2) g in group A, (1.16 ± 0. 0 1 1) g in group B, and group C (0 .95 0 ± 0. 0 0 9) g, the difference was significant (P <0. 05); 8 weeks after operation, A group was (2.20 ± 0.505) g in group B was (1.61 ± 0.103) g and group C was (1.230 ± 0.014) g, the difference was significant (P <0.05) . Four weeks and eight weeks postoperatively, light microscope and electron microscope were used to observe the cross-sectional area and wall thickness of sciatic nerve myelin sheath. Group A was better than group B, and group B was better than group C. Conclusion The target intramuscular injection of mecobalamin can promote the regeneration of the peripheral nerve, and the high dose of mecobalamin can better exert its nutritional induction on the injured nerve. The value of