水稻蜡质基因引导区的两个SSR序列与直链淀粉含量的相关性

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分析了蜡质基因引导区的两个简单重复序列(SSR)(CT)n和(AATT)n在74份水稻材料中的多态性及其与直链淀粉含量(AC)的关系。这些材料包括了籼稻(OryzasativaL.ssp.indica)、粳稻(O.sativassp.japonica)和普通野生稻(O.rufipogon),其AC值覆盖了栽培稻AC分布的整个范围。以(CT)n作标记检测到8个等位基因,粳稻品种趋于含有重复数目较多(n≥16)的等位基因,重复次数较少(n≤14)的等位基因只出现在籼稻中。(AATT)n检测到2个等位基因,野生稻中少数植株表现出杂合性。分析表明AC与这两个SSR序列基因型高度相关,高AC(>22.0%)品种具有(CT)重复次数较少(n≤14)的等位基因;相反,除了糯米外,所有低或者中等AC的品种都有(CT)重复数较多(n≥16)的等位基因。具有重复次数较多的(AATT)6等位基因的品种多为高AC,具有重复次数较少的(AATT)5等位基因的品种多为低或中等AC。不同SSR基因型品种间AC差异极显著。虽然目前还不能确定这两个SSR序列在直链淀粉合成中的直接功能,SSR变异与AC间近乎完全的相关性可作为分子标记直接用于水稻的品质改良。
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