论文部分内容阅读
目的掌握汉坦病毒(HV)宿主动物及基因型,为肾综合征出血热(HFRS)防制提供科学依据。方法居民区采用笼夜法捕鼠,野外采用夹夜法捕鼠,鼠肺组织样品用直接免疫荧光法检测HV抗原,用RT-PCR法检测HV核酸及分型。结果2006年在云南省泸西、寻甸、永胜和玉龙县共捕到鼠类7属12种690只,其中居民区捕到5属6种304只,以黄胸鼠和褐家鼠为优势鼠种;野外捕到7属12种386只,以高山姬鼠为优势鼠种。检测鼠肺624份,HV抗原阳性31份,带毒率4.97%(31/624),其中居民区鼠带毒率为2.17%(6/277),带毒鼠种为褐家鼠和黄胸鼠;野外鼠带毒率为7.20%(25/347),带毒鼠种为高山姬鼠、大绒鼠、中华姬鼠和黑腹绒鼠。HV核酸阳性21份,其中汉城型阳性3份(褐家鼠1份,大耳姬鼠1份,巢鼠1份),汉滩型阳性18份,(黄胸鼠2份,高山姬鼠7份,大绒鼠7份,黑腹绒鼠1份,巢鼠1份)。结论调查地区存在以褐家鼠为主要传染源的家鼠型HFRS疫源地,也存在以高山姬鼠和大绒鼠为主要传染源的野鼠型HFRS疫源地。HTN型病毒疫源地具有多种宿主动物的分布特点。
Objective To master the host animal and genotype of Hantavirus (HV) and provide a scientific basis for the prevention and treatment of hemorrhagic fever with renal syndrome (HFRS). Methods The residential area was caged by night-cage method. The wild-caught falciparum was used in the field. The HV antigen was detected by direct immunofluorescence and the HV nucleic acid was detected by RT-PCR. Results In 2006, 690 species of 12 species and 6 species of 7 genera were caught in Luxi, Xundian, Yongsheng and Yulong Counties, of which 304 were 5 genera and 6 species, Dominant species of rodents; wild catches 7 genus 12 species of 386, alpine Apodemus dominant mouse species. 624 murine lungs were tested positive for HV antigen, with a positive rate of 4.97% (31/624) for HV antigen, with 2.17% (6/277) virulence in residential areas, and Rattus norvegicus and yellow chest Rats in the wild were found to be 7.20% (25/347) virulent in the wild, while Apodemus agrarius, Plutella xylostella, Apodemus agrarius, 21 were positive for HV nucleic acid, of which 3 were positive for Seoul type (1 for Rattus norvegicus, 1 for Apodemus agrarius, 1 for nests), 18 for Hantaan type (2 for Rattus flavipectus, 7 parts of big-eared rat, 1 part of black-eared cashmere, 1 part of hamster). Conclusions There are foci of HFRS originating in Rattus norvegicus in the surveyed areas. There are also HFRS foci in the area of HFRS. HTN virus foci have a variety of host animal distribution characteristics.