Expression and Significance of Bcl-2, Bax, Fas and Caspase-3 in Different Phases of Human Hemangioma

来源 :华中科技大学学报(医学英德文版) | 被引量 : 0次 | 上传用户:yaoyaoyy1188
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
The relationship between Bcl-2, Bax, Fas, caspase-3 and development of hemangioma and the molecular mechanism was investigated. By using immunohistochemical S-P method, proliferating cell nuclear antigen was detected. According to the classification of Mulliken in combination with PCNA expression, 27 cases were identified as proliferating hemangioma and 22 cases as involutive hemangioma. Five normal skin tissues around the tumor tissue served as controls. By using immunohistochemical technique, the expression of Bcl-2, Bax, Fax and Caspase-3 was detected. The cells expressing Bcl-2, Bax, Fax and cappase-3 were identified as hemangioma endothelia by immunohistochemical staining of Ⅷ factor. The average absorbance (A) and average positive area rate of Bcl-2, Bax, Fas and caspase-3 expression were measured by using HPIAS-2000 imaging analysis system. The results showed that the expression of Bcl-2 in the endothelia of proliferating hemangioma was significantly higher that in involutive degenerative hemangioma endothelia and vascular endothelia of normal skin tissue (P<0.01). The expression of Bax, Fas and Caspase-3 in the endothelia of involutive hemangioma was obviously higher than in the endothelia of proliferating hemangioma and normal skin tissue (P<0.01). The expression of BAx and Fas in endothelia of proliferating hemangioma was higher than in those of normal skin tissue (P<0.05). It was suggested that Bcl-2,Bax, Fas and caspase-3 might be involved in the development and involution of hemangioma. Bcl-2 could promote the growth of hemangioma by inhibiting apoptosis of endothelia. Bax, Fas and caspase-3 promote the switch of hemangioma from proliferation to involution by inducing the apoptosis of hemangioma endothelia.
其他文献
Apramycin is unique in the aminoglycoside family due to its octodiose moiety. However, either the biosynthesis process or the precursors involved are largely un
Some extremal problems between the generalized Hua domain of the first type and the unit ball are studied. The extremal mapping and extremal value in explicit f
Nanoporous silica with unusual interior composite cells was synthesized with synthetic block copolypeptide Phe20-b-PBLG50 as template for the first time. Anilin
This article provides a framework e within which the revenue-sharing in mobile value-added services can be analyzed.It shows that the revenue-sharing ratio betw
The use of a CO2 laser system for fabrication of microfluidic chip on polymethyl methacrylate (PMMA) is presented to reduce fabrication cost and time of chip. T
The testing of thirteen reinforced concrete (RC) beams strengthened by epoxy-bonded glass fiber reinforced plastic plate (GFRP) shows that the RC beam and the G
北京谱仪(BESⅢ)采用阻性板室(Resistive Plate Chambers)作为其muon探测器,总使用面积达1200m2.为保证探测器的质量,每一块阻性板室都要求通过宇宙线测试.宇宙线测试系统使
Porous chitosan microspheres with diameters ranging from 180 μm to 280 μm were successfully prepared, using an anti-phase suspension method combined with temp
This paper studies the adsorption of diethyl phthalate (DEP,an environmental hormone) on the surface of nanoscale TiO2, effects of pH value of solutions, initia
The biochemical degumming and chemical degumming were employed to process apocynum venetum, and indices values for the residual gum content, fineness, strength