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应用Hungate厌氧技术,从大庆油田聚合物配注站的母液罐中分离到一株水溶性超高分子量聚丙烯酰胺(HPAM)的降解菌株I8,该菌株为短杆状,G-,黑色圆形菌落,最适温度为38℃,最佳pH值为7.8,具有硫酸盐还原功能,产H2S气体,兼性厌氧.研究表明,该菌株能以HPAM为唯一碳源,降解侧链,部分官能团发生改变;浓度为600mg/L时,20d菌株生物降解率为63.17%,其溶液粘度下降效果显著.16S rDNA序列与Enterobacter cloacae(ECL251469)的相似性为98%,通过形态、生理生化、G+C含量以及16S rDNA序列鉴定,初步鉴定可能为肠杆菌属的一个新种,暂时命名为Enterobacter HPAM Degraded Bacteria I8,I8菌株的分离为HPAM的生物降解提供了新的微生物资源.图4,参14.
Using Hungate anaerobic technology, a degradable strain I8 of water-soluble ultra-high molecular weight polyacrylamide (HPAM) was isolated from the mother liquor tank of polymer injection station in Daqing Oilfield. The strain was short rod, G- and black circle Shaped colonies, the optimum temperature was 38 ℃, the optimum pH was 7.8, with sulfate reduction function, producing H2S gas, facultative anaerobic. Studies have shown that this strain can use HPAM as the sole carbon source, degradation of side chains, part of The biodegradability of the 20d strain was 63.17% at 600mg / L, and its solution viscosity decreased significantly.The similarity of 16S rDNA sequence to Enterobacter cloacae (ECL251469) was 98% + C content and 16S rDNA sequence identification, preliminary identification may be a new species of Enterobacter, temporarily named Enterobacter HPAM Degraded Bacteria I8, Isolation of I8 strain provides a new microbial resources for the biodegradation of HPAM Figure 4, ginseng 14.