目的:检测Wnt通路靶基因PISD在结肠直肠细胞及组织标本中的表达情况及其与临床病理的关系。方法:应用Western印迹法测定结肠直肠癌细胞株中PISD的蛋白表达水平,应用免疫组化法测定PISD在组织标本中的表达情况及其与临床病理的关系。结果:Western印迹法结果显示PISD在7种结肠直肠癌细胞株中均呈阳性表达,免疫组化结果显示PISD在结肠直肠癌组织中表达阳性,灵敏度为62.9%。结论:PISD在结肠直肠癌细胞及组织中表达上调,但与肿瘤的转移无明显相关。 OBJECTIVE: To detect the expression of PISD, a target gene of Wnt pathway, in colorectal cells and tissues and its relationship with clinical pathology. Methods: Western blotting was used to determine the protein expression of PISD in colorectal cancer cell lines. The expression of PISD in tissue samples and its relationship with clinicopathological parameters were determined by immunohistochemistry. Results: Western blotting showed that PISD was positive in all seven colorectal cancer cell lines. Immunohistochemical results showed that PISD was positive in colorectal cancer with a sensitivity of 62.9%. Conclusion: PISD is up-regulated in colorectal cancer cells and tissues, but it has no significant correlation with tumor metastasis.