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目的评估登革热病毒(DENV)对广州地区血液安全的影响。方法采用分层随机抽样的方法抽取2014年9月15日至10月15日在广州血液中心献血的无偿献血者3 000名,ELISA法检测其血清中DENV IgM抗体并按年龄和性别比较阳性率差异。IgM检测结果为阳性者进行DENV RNA检测。结果 3 000名献血者检出DENV Ig M抗体阳性标本71份,阳性率为2.4%。其中男性44名,女性27名,阳性率分别为2.1%及2.9%,男女阳性率差异无统计学意义(χ2=1.483,P=0.223);18~岁年龄组、25~岁年龄组、35~岁年龄组和45~60岁年龄组献血者抗DENV Ig M抗体阳性率分别为2.2%、2.5%、2.0%和3.1%,差异无统计学意义(χ2=1.209,P=0.751)。71名DENV Ig M阳性献血者中检出DENV RNA阳性1名,病毒滴度为9.44E2 copies/ml,阳性率为0.03%。结论携带DENV的献血者对血液安全应当引起重视,在DENV流行区或者流行期间,应当建立相应的筛查方法,并把其纳入献血者筛查项目。
Objective To evaluate the impact of dengue virus (DENV) on blood safety in Guangzhou. Methods Thirty thousand blood donors were donated in blood from Guangzhou Blood Center from September 15 to October 15, 2014 by stratified random sampling method. The serum DENV IgM antibody was detected by ELISA and the positive rate was compared by age and sex difference. DENV RNA was detected with IgM test positive. Results 7100 DENV Ig M positive samples were detected in 3 000 blood donors, the positive rate was 2.4%. There were 44 males and 27 females, the positive rates were 2.1% and 2.9%, respectively. There was no significant difference between the male and female positive rates (χ2 = 1.483, P = 0.223); 18 to 25 years, 35 to 35 years The positive rates of anti-DENV Ig M antibody in the ~ ~ age group and the 45 ~ 60 age group were 2.2%, 2.5%, 2.0% and 3.1%, respectively, with no significant difference (χ2 = 1.209, P = 0.751). One of 71 DENV Ig M positive donors was positive for DENV RNA with a titer of 9.44E2 copies / ml and a positive rate of 0.03%. Conclusion Blood donors who carry DENV should pay more attention to blood safety. During the endemic or epidemic period of DENV, appropriate screening methods should be established and put into the blood donation screening project.