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目的:探讨TRIM72在游泳运动改善高脂饮食大鼠IR中的作用。方法:以SD大鼠为实验对象,随机分为4组:普通饮食对照组(C组)、普通饮食运动组(CE组)、高脂饮食IR模型组(H组)、高脂饮食运动组(HE组)。通过8周高脂饲料喂养建立IR大鼠动物模型,同时对大鼠实施无负重游泳运动干预。用正糖钳技术结合胰岛素抵抗指数(HOMA-IR)和胰岛素敏感指数(ISI)评价动物模型的建立;通过观察运动对高脂饮食IR大鼠脂质沉积、骨骼肌氧化应激水平、TRIM72和Akt表达水平的变化,探讨运动干预IR的机制。结果:1)8周高脂饮食喂养后,H组大鼠葡萄糖输注速率(GIR)显著下降(P<0.01),ISI水平显著下降(P<0.01),HOMA-IR水平显著增加(P<0.01),提示胰岛素抵抗大鼠建模成功。2)8周游泳干预后,与H组相比,HE组大鼠GIR显著增加(P<0.01),血清INS含量和HOMA-IR水平均显著降低(P<0.01),ISI水平显著增加(P<0.05或P<0.01);HE组大鼠骨骼肌脂质沉积减少,骨骼肌SOD和GSH-Px活性均显著增加(P<0.05,P<0.01),MDA和8-OH-d G含量均显著减少(P<0.05,P<0.01),骨骼肌TRIM72 mRNA和TRIM72蛋白表达水平均显著下降(P<0.05,P<0.01),骨骼肌p AktSer473磷酸化水平和Akt蛋白表达水平均显著增加(P<0.05,P<0.01),p IRS-1Ser307的磷酸化水平显著下降(P<0.01)。结论:8周游泳运动可以通过抑制氧化应激,减轻骨骼肌细胞受损伤程度,从而降低大鼠骨骼肌TRIM72表达水平,增强骨骼肌PI3-K/Akt信号转导,改善高脂饮食大鼠IR。
Objective: To investigate the role of TRIM72 in swimming improving the IR of high-fat diet rats. Methods: SD rats were randomly divided into 4 groups: normal diet control group (C group), normal diet exercise group (CE group), high fat diet IR model group (H group), high fat diet exercise group (HE group). The animal model of IR rats was established by feeding for 8 weeks with high-fat diet, meanwhile, the rats were subjected to non-weight-bearing swimming exercise intervention. The effects of exercise on the lipid deposition, skeletal muscle oxidative stress, TRIM72 and insulin resistance in IR rats of high-fat diet were observed by using positive glucose clamp technique combined with insulin resistance index (HOMA-IR) and insulin sensitivity index (ISI) Akt expression changes, to explore the mechanism of exercise intervention IR. Results: 1) After 8 weeks of high-fat diet, the GIR of H group was significantly decreased (P <0.01), ISI level was significantly decreased (P <0.01), HOMA-IR level was significantly increased 0.01), suggesting that insulin resistance rats model successful. 2) Compared with H group, GIR in HE group increased significantly (P <0.01), serum INS level and HOMA-IR level decreased significantly (P <0.01) and ISI level increased significantly (P <0.05 or P <0.01). The deposition of lipid in skeletal muscle of rats in HE group was decreased and the activities of SOD and GSH-Px in skeletal muscle were significantly increased (P <0.05, P <0.01) (P <0.05, P <0.01). The expressions of TRIM72 mRNA and TRIM72 protein in skeletal muscle were significantly decreased (P <0.05, P <0.01), and the phosphorylation of p AktSer473 and Akt protein in skeletal muscle were significantly increased P <0.05, P <0.01), phosphorylation of p IRS-1Ser307 significantly decreased (P <0.01). Conclusion: 8-week swimming exercise can reduce the damage of skeletal muscle cells by inhibiting oxidative stress, thereby reducing TRIM72 expression in skeletal muscle, enhancing PI3-K / Akt signaling in skeletal muscle and improving IR .