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目的近年来研究发现白藜芦醇(Resveratrol,RSV)具有抗氧化、清除自由基、减轻缺血再灌注损伤等作用。将其与减轻缺血再灌注损伤的主要手段——缺血后处理(Ischemic Postconditioning,IPO)相结合,共同作用于体外培养的H9C2心肌细胞受损模型。方法采用免疫细胞化学、western blot以及RT-PCR等方法,观察这种共同作用对体外培养的H9C2心肌细胞受损模型细胞增殖的影响。结果 p38表达量随着RSV+IPO的干预和时间的延长而增加;通过直观和检测cyclinA2发现H9C2细胞数量因加入干预手段有所增加,cyclinA2的表达量也有相同趋势。结论研究结果表明,该方法不仅可以抑制细胞凋亡,更具有促进细胞增殖的作用。
OBJECTIVE: In recent years, Resveratrol (RSV) has been shown to have antioxidant, scavenging free radicals, and to reduce the effects of ischemia-reperfusion injury. Combined with Ischemic Postconditioning (IPO), a primary measure to reduce ischemia-reperfusion injury, H9C2 cardiomyocytes injury model was established. Methods Immunocytochemistry, western blot and RT-PCR were used to observe the effect of this interaction on the proliferation of H9C2-injured cardiomyocytes cultured in vitro. Results The expression of p38 was increased with the intervention of RSV + IPO and the prolongation of time. The number of H9C2 cells increased with the increase of intervention and the expression of cyclinA2 also showed the same trend through visualization and detection of cyclinA2. Conclusion The results show that this method can not only inhibit apoptosis, but also promote cell proliferation.