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目的:观察三七对酒精性大鼠血清TNF-α、IL-6、IL-8水平的影响。方法:SD雄性大鼠随机分为正常组,模型组、三七高、低剂量组和硫普罗宁组,连续14周建立酒精性肝病模型。在模型制备同时,每天下午分别灌服给药,连续14周。ELISA法检测肿瘤坏死因子(TNF-α),放射免疫法测定血清IL-6、IL-8水平。常规HE及Masson染色,光镜观察肝组织的脂肪变、炎症化程度。结果:酒精性肝病模型组大鼠肝组织脂肪变及炎症程度计分、血清TNF-α、IL-6、IL-8水平明显增高(P<0.01,P<0.05);三七高、低剂量组,硫普罗宁组大鼠肝组织脂肪变及炎症程度、血清TNF-α、IL-6、IL-8水平较模型组明显减轻(P<0.01,P<0.05)。相关分析显示:血清TNF-α水平与肝组织炎症程度计分呈正相关(r=0.60,P<0.01),血清IL-6水平与肝组织炎症程度计分呈正相关(r=0.47,P<0.01)血清IL-8水平与肝组织炎症程度计分呈正相关(r=0.59,P<0.01)。结论:三七可明显减轻酒精性肝病大鼠肝组织脂肪变和炎症程度。三七能调节细胞因子网络,抑制ALD大鼠血清TNF-α、IL-6、IL-8的生成;这可能是其有效防治酒精性肝病的发生发展的重要机制之一。
Objective: To observe the effects of Panax notoginseng on levels of serum TNF-α, IL-6 and IL-8 in alcoholic rats. METHODS: SD male rats were randomly divided into normal group, model group, high-dose group and low-dose group, and tiopronin group. Alcoholic liver disease model was established for 14 consecutive weeks. Simultaneously with the model preparation, they were administered daily in the afternoon for 14 consecutive weeks. ELISA was used to detect tumor necrosis factor (TNF-α). Radioimmunoassay was used to measure serum IL-6 and IL-8 levels. Routine HE and Masson staining were used to observe the degree of steatosis and inflammation in liver tissue by light microscopy. RESULTS: The liver tissue steatosis and inflammation scores, serum TNF-α, IL-6 and IL-8 levels in the alcoholic liver disease model group were significantly higher (P<0.01, P<0.05); Panax notoginseng high and low dose In the tiopronin group, the levels of steatosis, inflammation, serum TNF-α, IL-6 and IL-8 levels in the tiopronin group were significantly lower than those in the model group (P<0.01, P<0.05). Correlation analysis showed that serum TNF-α levels were positively correlated with liver tissue inflammation scores (r=0.60, P<0.01). Serum IL-6 levels were positively correlated with liver tissue inflammation scores (r=0.47, P<0.01). ) Serum IL-8 levels were positively correlated with liver tissue inflammation scores (r=0.59, P<0.01). Conclusion: Panax notoginseng can significantly reduce the degree of liver steatosis and inflammation in rats with alcoholic liver disease. Notoginseng can regulate the cytokine network and inhibit the production of serum TNF-α, IL-6 and IL-8 in ALD rats. This may be one of the important mechanisms for effective prevention and treatment of alcoholic liver disease.