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目的 建立固定中性粒细胞－ＥＬＩＳＡ，分析血清中抗中性粒细胞胞浆抗体（ＡＮＣＡ）对溃疡性结肠炎（ＵＣ）的诊断价 值。方法 采用固定中性粒细胞－ＥＬＩＳＡ法检测 ２５份 ＵＣ血清、２６份细菌性结肠炎血清、１５份混合类型腹泻血清和 ５０份正 常对照血清中的 ＡＮＣＡ，对各组血清的吸光度（Ａ）值进行统计分析。结果 ＵＣ组 ＡＮＣＡ与其他对照组 ＡＮＣＡ之间的差异非 常显著（Ｐ＜０．００５）。ＵＣ组Ａ值超过正常对照组Ａ值２s以上病例达８０％，诊断特异性达８９％。结论 ＵＣ血清中存在有诊断 价值的 ＡＮＣＡ，固定中性粒细胞一ＥＬＩＳＡ可作为血清 ＡＮＣＡ检测的重要手段。
Objective To establish fixed neutrophil-ELISA for the diagnosis of ulcerative colitis (UC) in serum anti-neutrophil cytoplasmic antibody (ANCA). Methods ANCA in sera of 25 patients with UC, 26 patients with bacterial colitis, 15 mixed type diarrhea and 50 normal controls were detected by fixed neutrophil-ELISA. The serum absorbance (A) of each group conduct statistical analysis. Results The difference between ANCA in UC group and ANCA in other control group was significant (P <0.005). The value of A in UC group was 80% higher than that in normal control group A value of 2s or more, and the diagnostic specificity was 89%. Conclusion There is a diagnostic value of ANCA in UC serum, and neutrophil-immobilized ELISA can be used as an important measure of serum ANCA.