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目的:研究永久性大脑局灶缺血后血管内皮生长因子(VEGF)、angiopoietin-1(Aug-1)、Aug-2、Tie-1和Tie-2在C57BL/6小鼠大脑皮层的表达.方法:采用半定量逆转录聚合酶链反应(RT-PCR)研究VEGF、Aug-1、Aug-2、Tie-1和Tie-2 mRNA表达的变化.采用免疫组织化学法研究VEGF和Aug-2蛋白的表达.结果:在正常小鼠大脑皮层VEGF、Aug-1、Aug-2、Tie-1和Tie-2 mRNA表达水平很低.当大脑中动脉阻断(MCAO)后,在梗塞皮层VEGF、Ang-2和Tie-2 mRNA表达显著增加,并且可维持到第7天.但Ang-1和Tie-1 mRNA表达在梗塞皮层无明显变化.在正常小鼠大脑皮层,几乎观察下到VEGF和Ang-2蛋白的表达.MCAO后8小时Ang-2蛋白发达在皮层梗塞区明显增加,1天后可在梗塞灶周边区看到Ang-2蛋白增加;而VEGF蛋白则在MCAO后2小时在皮层梗塞区即显著升高,1天后在梗塞灶周边区可观察到VEGF蛋白增加.不论在皮层梗塞区还是在梗塞灶周边区,Ang-2和VEGF免疫阳性都限于内皮细胞和胶质样细胞.结论:大脑局灶缺血后Ang-2和VEGF表达增加,可能有利于缺血大脑皮层的血管新生.
AIM: To investigate the expression of vascular endothelial growth factor (VEGF), angiopoietin-1 (Aug-1), Aug-2, Tie-1 and Tie-2 in cerebral cortex of permanent C57BL / 6 mice after permanent focal cerebral ischemia. Methods: The changes of mRNA expression of VEGF, Aug-1, Aug-2, Tie-1 and Tie-2 were studied by semi-quantitative reverse transcriptase polymerase chain reaction (RT- Protein expression.Results: The expression of VEGF, Aug-1, Aug-2, Tie-1 and Tie-2 mRNA in the normal mouse cerebral cortex was low.When MCAO was occluded, , Ang-2 and Tie-2 mRNA expression increased significantly, and can be maintained until day 7. However, Ang-1 and Tie-1 mRNA expression in the infarct cortex no significant changes in the normal mouse cerebral cortex, almost observed to VEGF And Ang-2 protein expression.At 8 hours after MCAO, the expression of Ang-2 protein was significantly increased in the infarcted area of the cortex, and the increased Ang-2 protein was seen in the peripheral area of the infarcted area one day later, while the VEGF protein was increased at 2 hours Cortical infarction area was significantly increased, one day after infarction in the peripheral area can be observed in VEGF protein increased in both cortical infarct area or peripheral infarction area, Ang-2 and VEGF-positive They are limited to endothelial cells and glial-like cells Conclusion: Focal brain increased Ang-2 and VEGF expression after ischemia, may facilitate angiogenesis in ischemic cerebral cortex.