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目的 建立肠出血性大肠杆菌O15 7:H7感染小鼠动物模型。方法 选用离乳小鼠随机分组 ,先以丝裂霉素和萘啶酮酸处理 ,提高小鼠对肠出血性大肠杆菌O15 7的敏感性 ,再分别以不同剂量口服接种肠出血性大肠杆菌O15 7:H7EDL933W ,进行临床和病理学检查。结果 利用对O15 7不易感的小鼠 ,复制出人类感染O15 7所出现的主要病理变化和部分临床症状 ,初步建立了动物模型。讨论 该动物模型对探索肠出血性大肠杆菌O15 7:H7的感染机制、毒力因子的作用有重要意义 ,并为O15 7:H7的疫苗侯选株安全性的评价打下初步基础。
Objective To establish an animal model of enterohemorrhagic Escherichia coli O15 7: H7 infection in mice. Methods The mice were randomly divided into three groups. Mitomycin and nalidixic acid were used to improve the sensitivity of the mice to enterohemorrhagic Escherichia coli O15 7. Oral hemorrhagic Escherichia coli O15 7: H7EDL933W for clinical and pathological examination. Results The primary pathological changes and some clinical symptoms of human infection O157 were replicated in mice that were not susceptible to O157. The animal model was initially established. The animal model discussed to explore the mechanism of infection of enterohemorrhagic Escherichia coli O15 7: H7, the role of virulence factors is important, and lay the preliminary foundation for the safety evaluation of O15 7: H7 vaccine candidate strains.