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用ELISA方法测定95例SARS患者急性期、恢复期血清细胞因子水平,用流式细胞仪检测该组患者急性期、恢复期淋巴细胞亚群,并分析细胞因子水平和淋巴细胞亚群变化的关系。结果发现,IL-10和TGF-β在观察期(急性期和恢复期)持续升高,急性期CD4+和CD8+T细胞显著减少,恢复期患者外周血CD8+记忆T细胞减少36.78%。IL-10和TGF-β升高与淋巴细胞变化具有统计学相关。结果提示,SARS病毒感染抑制宿主的细胞免疫功能,引起急性期CD4+和CD8+T细胞显著降低和恢复期的免疫记忆细胞减少。而IL-10和TGF-β过表达可能在SARS免疫病理中起重要作用。
The levels of serum cytokines in acute and convalescent phase of 95 SARS patients were measured by ELISA. The acute and convalescent lymphocyte subsets in this group were detected by flow cytometry. The relationship between cytokine levels and changes of lymphocyte subsets . The results showed that IL-10 and TGF-βwere continuously increased in the observation period (acute phase and convalescent phase), the number of CD4 + and CD8 + T cells in acute phase decreased significantly, and the percentage of CD8 + memory T cells in convalescent phase decreased 36.78%. Elevated IL-10 and TGF-β were correlated with lymphocyte changes. The results suggest that SARS virus infection inhibits the host’s cellular immune function, causing a significant decrease in acute CD4 + and CD8 + T cells and convalescent immune memory cells. However, overexpression of IL-10 and TGF-β may play an important role in the immunopathology of SARS.