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目的:探讨转化生长因子β1(TGF-β1)和转化生长因子βRⅡ(TGFβRⅡ)的表达与肝癌临床病理因素的关系,了解TGF-β1和TGFβRⅡ在肝癌浸润、转移中的作用。方法:用免疫组化方法(S-P法)检测原发性肝细胞肝癌和癌旁组织57例中TGF-β1和TGFβRⅡ的表达水平。结果:TGF-β1在肝癌组织中的阳性表达47例(82.46%)显著高于癌旁组织38例(66.67%)(P<0.05);TGFβRⅡ在肝癌组织中的阳性表达20例(35.09%),显著低于癌旁组织48例(84.21%)(P<0.05)。TGF-β1在有转移的患者中的表达率为(92.31%)明显高于无转移的患者(74.19%)(P<0.05),而TGFβRⅡ在有包膜浸润、转移、组织分化Ⅲ-Ⅳ的患者中染色较浅且阳性表达率明显下降,分别为19.52%、19.23%和7.14%,明显低于相对应组50.00%、49.39%和62.07%的阳性表达率(P<0.01)。结论:TGF-β1过度表达和TGFβRⅡ表达下降可能增强了肝癌细胞的浸润、转移。
Objective: To investigate the relationship between the expression of transforming growth factor β1 (TGF-β1) and transforming growth factor βRⅡ (TGFβRⅡ) and the clinicopathological factors of hepatocellular carcinoma (HCC), so as to understand the role of TGF-β1 and TGFβRII in the invasion and metastasis of hepatocellular carcinoma. Methods: The expressions of TGF-β1 and TGFβRⅡ were detected by immunohistochemistry (S-P method) in 57 cases of primary hepatocellular carcinoma and adjacent non-cancerous tissues. Results: The positive expression of TGF-β1 in hepatocellular carcinoma was significantly higher than that in adjacent tissues (47 cases, 82.46%, 38 cases, 66.67%, P <0.05) , Significantly lower than the 48 cases of paracancerous tissues (84.21%) (P <0.05). The expression of TGF-β1 in patients with metastasis (92.31%) was significantly higher than that in patients without metastasis (74.19%) (P <0.05), whereas the expression of TGFβRⅡ in patients with metastasis and invasion The patients were stained lightly and the positive expression rates were significantly decreased, which were 19.52%, 19.23% and 7.14% respectively, which were significantly lower than those of the corresponding groups 50.00%, 49.39% and 62.07% (P <0.01). Conclusion: The overexpression of TGF-β1 and the decrease of TGFβRⅡ expression may enhance the invasion and metastasis of hepatocellular carcinoma cells.