论文部分内容阅读
本文应用压电沉淀吸附法(PPAA)开展了对毛果芸香碱的测定。在柠檬酸缓冲液(CBS)中,用四苯硼钠(TPB)与盐酸毛果芸香碱反应生成稳定的胶体溶液,胶体颗粒吸附于石英晶体表面,使石英晶体的谐振频率发生改变(△F)。考察了CBS浓度及pH值、TPB浓度和温度等实验条件对ΔF的影响,测定了压电传感器对毛果芸香碱的响应特性。结果表明,盐酸毛果芸香碱在0.034—0.758mmol/L范围,△F与毛果芸香碱浓度间呈线性关系。将此传感器用于实际样品及硝酸毛果芸香碱滴眼液中毛果芸香碱含量的测定,结果分别与电位滴定法测定结果及标示量相符。
In this paper, the application of piezoelectric precipitation adsorption (PPAA) carried out the determination of pilocarpine. In citric acid buffer (CBS), a stable colloidal solution was formed by the reaction of tetraphenylboron sodium (TPB) with pilocarpin hydrochloride. The colloidal particles adsorbed on the quartz crystal surface changed the resonant frequency of the quartz crystal (△ F). The effects of experimental conditions such as CBS concentration, pH value, TPB concentration and temperature on ΔF were investigated. The response of piezoelectric sensor to pilocarpine was measured. The results showed that pilocarpine hydrochloride in the range of 0.034-0.758mmol / L, △ F and the concentration of pilocarpine showed a linear relationship. The sensor was used to determine the content of pilocarpine in the actual samples and pilocarpine nitrate eye drops. The results were in good agreement with those obtained by potentiometric titration.