目的:研究海南黎族人群血管紧张素转化酶(ACE)基因多态性与冠心病(CHD)关系。方法:采用聚合酶链反应(PCR)技术,对海南黎族150例CHD患者(CHD组)和150例正常人(正常对照组)的ACE基因插入/缺失(I/D)多态性进行检测,观察DD、DI、II基因型频率及等位基因频率,并对所有普通PCR定为DD型的样本进行插入特异性PCR检测,以减少误分型率。结果:CHD组DD、DI、II基因的频率分别是24.7%、32.7%、42.6%;D及I等位基因频率分别为41.0%、59.6%;正常对照组DD、DI、II基因型频率分别为14.0%、44.0%、42.0%,D及I等位基因频率分别为36.0%、64.0%;2组之间的DD、DI、II基因频率及D、I等位基因频率,均差异有统计学意义(P<0.05)。结论:ACEI/D多态性与黎族CHD有显著关联,是海南黎族CHD的主要致病基因。早期应用ACE抑制剂、血管紧张素受体拮抗剂防治,有十分重要意义。 Objective: To study the relationship between angiotensin-converting enzyme (ACE) gene polymorphism and coronary heart disease (CHD) in Hainan Li population. Methods: ACE gene insertion / deletion (I / D) polymorphism was detected in 150 CHD patients (CHD group) and 150 healthy controls (normal control group) in Hainan Li ethnic group by polymerase chain reaction (PCR) The frequencies of DD, DI, and II genotypes and allele frequencies were observed. Insert PCR-specific PCR was performed on all samples with DD as the normal PCR to reduce the misdiagnosis rate. Results: The frequencies of DD, DI and II genes in CHD group were 24.7%, 32.7% and 42.6%, respectively. The frequencies of D and I allele were 41.0% and 59.6% respectively. The frequencies of DD, DI and II genotypes in CHD group were Were 14.0%, 44.0% and 42.0%, respectively. The frequencies of D and I allele were 36.0% and 64.0% respectively. There was statistical difference between the two groups in the frequencies of DD, DI, II and D, I alleles Significance (P <0.05). Conclusion: There is a significant association between ACEI / D polymorphism and Li CHD, which is the main pathogenic gene of CHD in Hainan Li ethnic group. Early application of ACE inhibitors, angiotensin receptor antagonist prevention and treatment, is of great significance.