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目的 建立新生大鼠铅损伤动物模型 ,用于研究铅对发育脑的损害。方法 选取 3窝新生Wistar大鼠 ,随机分成正常对照组、低沿组和高铅组。分别于出生 0、2、4、6、8、10、12、14d给予腹腔注射醋酸铅溶液 0 1mL ,注射剂量分别为 0、2 0、6 0mg kg(按出生体重计算 )。各鼠分组不分窝 ,均由母鼠哺乳。结果 第 17d断头处死各鼠 ,检测血铅值分别为 :对照组 3 5± 6 6 μg L、低铅组 4 10 8± 4 6 6 3μg L、高铅组均 >6 5 0 μg L ,正常组在正常范围内 ,低铅组已达中度铅中毒 ,而高铅组已达重度铅中毒。结论 新生大鼠腹腔注射醋酸铅溶液建立动物铅损伤模型是一种简便、可靠、易于控制的方法 ,可用于铅对发育脑损伤的研究
OBJECTIVE: To establish animal models of lead injury in neonatal rats and to study the effects of lead on developing brain damage. Methods Three newborn Wistar rats were randomly divided into normal control group, low-incision group and high-lead group. At 0, 2, 4, 6, 8, 10, 12 and 14 days of birth, 0.1 ml of lead acetate solution was intraperitoneally injected. The injected doses were 0, 20 and 60 mg kg (calculated by birth weight). The mice were divided into groups regardless of litter, were nursing mothers. Results The rats were sacrificed on the 17th day after the decapitation. The blood lead levels were 35 ± 6 6 μg in the control group, 4 10 8 ± 4 6 6 3 μg L in the low lead group and> 650 μg in the lead group. The normal group within the normal range, low lead group has reached moderate lead poisoning, and high lead group has reached severe lead poisoning. Conclusion The animal model of lead injury induced by intraperitoneal injection of lead acetate in neonatal rats is a simple, reliable and easy-to-control method that can be used to study the effects of lead on developing brain injury