目的 :构建编码乙型肝炎病毒 (HBV)表面蛋白S的重组质粒pCR 3 .1 S,将之直接肌肉注射balb/c小鼠 ,观察小鼠HBV特异的免疫应答。方法 :以ELISA法检测小鼠血清 ,3H TdR掺入法测定淋巴细胞增殖 ,51 Cr 4h释放法检测淋巴细胞杀伤功能。结果 :与空载体对照组相比较 ,基因疫苗诱发小鼠产生良好的抗HBs反应及HBV特异的细胞免疫应答 (P <0 .0 5)。结论 :基因疫苗pCR 3 .1 S可诱导balb/c小鼠产生全面的免疫应答 OBJECTIVE: To construct a recombinant plasmid pCR3.1S encoding hepatitis B virus surface protein S and directly inoculate it into balb / c mice to observe the specific immune response of mice. Methods: The serum of mice was detected by ELISA and the lymphocyte proliferation was measured by 3H TdR incorporation method. The cytotoxicity of lymphocytes was detected by 51 Cr 4h release method. Results: Compared with the control group, the gene vaccine induced good anti-HBs response and HBV-specific cellular immune response (P <0.05). CONCLUSIONS: The gene vaccine pCR3.1S induces a complete immune response in balb / c mice