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毕赤酵母表达系统作为一种新型酵母表达系统 ,它既有原核生物的特点 ,又有真核生物的特性 ,可以对目的蛋白质进行糖基化、二硫键形成等翻译后修饰。在过去的 2 0年中已经有 2 0 0多种不同蛋白在毕赤酵母中实现了成功表达〔1〕,其中许多已被广泛应用于临床诊断治疗或科研工作中。随着一系列新型酵母表达载体及菌株的构建及发酵技术的不断提高 ,毕赤酵母越来越吸引着科学家的关注 ,毕赤酵母己被发展成为一种细胞生物学研究及基因工程生产的模式真核生物。本文总结了毕赤酵母的载体与菌株构建及发酵技术和糖基化等方面近年的主要研究进展
As a new yeast expression system, Pichia pastoris expression system has the characteristics of prokaryotes and eukaryotes, and can be used for post-translational modification of glycosylation, disulfide bond formation and the like of the target protein. In the past 20 years, more than 200 different proteins have been successfully expressed in Pichia pastoris [1], many of which have been widely used in clinical diagnosis and treatment or scientific research. With the development of a series of new yeast expression vectors and strains and the continuous improvement of fermentation techniques, Pichia pastoris attracts more and more scientists’ attention. Pichia has been developed into a model for cell biology research and genetic engineering production Eukaryotes. This review summarizes recent advances in vector and strain construction of Pichia pastoris, fermentation techniques and glycosylation