论文部分内容阅读
为了解中亚热带森林土壤的真菌多样性,利用PCR-DGGE分子指纹图谱技术对武夷山国家级自然保护区和建瓯万木林自然保护区内的米槠(Castanopsis carlesii)天然林土壤真菌进行研究。结果表明,中亚热带地区森林土壤真菌群落丰富,土壤真菌的多样性指数、丰富度指数和均匀度指数分别为3.37~3.80、26~29和0.977~0.984。经测序及同源性比对,真菌以担子菌类(Basidiomycota)最多,占总数的35.9%;其次为子囊菌类(Ascomycota),占总数的15.4%;接合菌类(Zygomycota)和半知菌类(Deuteromycota)分别占总数的10.3%和7.7%。主成分分析表明,地点和土层深度共同影响真菌群落组成,其中由地点引起的真菌群落组成差异大于土层深度。土壤真菌多样性指数与土壤铵态氮呈显著正相关,与土壤碳氮比呈显著负相关;丰富度指数和均匀度指数与土壤因子间没有显著的相关性,因此,土壤碳、氮含量是影响土壤真菌群落结构的重要因子。
In order to understand the fungal diversity of soils in the subtropical forest, the soil fungi of Castanopsis carlesii natural forest in Wuyishan National Nature Reserve and Jian’ou Wanmulin Nature Reserve were studied by PCR-DGGE molecular fingerprinting. The results showed that the fungal communities in the forest soils in the subtropical region were rich, and the diversity index, richness index and evenness index of soil fungi were 3.37-3.80, 26-29 and 0.977-0.984, respectively. After sequencing and homology comparison, the fungi were Basidiomycota, accounting for 35.9% of the total, followed by Ascomycota, accounting for 15.4% of the total. Zygomycota and Candida, Deuteromycota accounted for 10.3% and 7.7% of the total, respectively. The principal component analysis showed that the location and depth of the soil together affected the composition of the fungal community, of which the composition of the fungal community caused by the location was greater than the depth of the soil. Soil fungal diversity index was positively correlated with soil ammonium nitrogen and negatively correlated with soil C and N ratios. There was no significant correlation between soil fungi index and evenness index. Therefore, soil carbon and nitrogen contents were Important factors affecting soil fungal community structure.