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目的:通过对2008年本实验室新确认140例HIV-1抗体阳性者WB带型、CD4+T细胞数的分析,了解不同人群不同病期免疫学、血清学变化特征。方法:应用免疫印迹试验(WB)、流式细胞仪检测HIV-1感染者体内抗体、CD4+T细胞数。结果:不同感染途径WB带型分布无明显差异(P>0.05),但CD4+T细胞数差异有统计学意义;年龄(age,A)≤40岁的HIV-1抗体阳性者体内全带型(9条带)与P55条带出现率分别明显高于A>40岁以上者;不同性别WB带型分布无明显差异(P>0.05),但CD4+T细胞计数之间差异有统计学意义;AIDS病人与HIV-1感染者的WB反应全带型、p55条带的出现率及CD4+T细胞数差异有统计学意义。结论:不同人群WB反应带型与CD4+T细胞数都有不同程度的差异,该项指标为追踪HIV-1传染源、传播途径及临床分期、疾病预后提供依据。
OBJECTIVE: To understand the immunological and serological changes in different stages of different populations by analyzing the number of WB bands and CD4 + T cells in 140 newly diagnosed HIV-1 antibodies in our laboratory in 2008. Methods: The number of antibody and CD4 + T cells in HIV-1 infected persons was detected by Western blotting (WB) and flow cytometry. Results: There was no significant difference in the distribution of WB bands between different routes of infection (P> 0.05), but the number of CD4 + T cells was significantly different. The age-range, A (≤40) HIV- (9 bands) and P55 bands were significantly higher than those of A> 40 years old respectively. There was no significant difference in the distribution of WB bands among different sexes (P> 0.05), but the difference of CD4 + T cell counts was statistically significant ; WB patients with HIV-1 infection and AIDS patients with full-band type, p55 band incidence and CD4 + T cell number difference was statistically significant. CONCLUSION: The differences of WB response type and CD4 + T cell count in different populations are different. This index provides the basis for tracing the source of HIV-1, the route of transmission and clinical stage, and the prognosis of the disease.