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目的探讨姜黄素对经转化生长因子β(TGF-β)诱导后人胚肺成纤维细胞TGF-βⅠ、Ⅱ型受体(TGF-βRⅠ、RⅡ)在人胚肺成纤维细胞中表达的影响,及其与特发性肺纤维化的关系。方法细胞分为正常组、姜黄素组、模型组、治疗组,采用RT-PCR、Western blotting法检测TGF-βRⅠ、RⅡ在人胚肺成纤维细胞的变化。结果模型组TGF-βRⅠ与RⅡ表达增强,治疗组与正常组、模型组相比,TGF-βRⅠ与RⅡ表达明显减弱。结论姜黄素可抑制人胚肺成纤维细胞的胶原沉积,在延缓肺纤维化形成中起一定作用,其作用机制可能是通过抑制TGF-βRⅠ与RⅡ的表达而实现的。
Objective To investigate the effect of curcumin on the expression of TGF-β Ⅰ and Ⅱ receptors (TGF-βRⅠ, RⅡ) in human embryonic lung fibroblasts after induced by transforming growth factor β (TGF-β) And its relationship with idiopathic pulmonary fibrosis. Methods The cells were divided into normal group, curcumin group, model group and treatment group. The changes of TGF-βRⅠ and RⅡ in human embryo lung fibroblasts were detected by RT-PCR and Western blotting. Results The expression of TGF-βRⅠand RⅡwere increased in model group, and the expression of TGF-βRⅠ and RⅡwere significantly decreased in treatment group compared with normal group and model group. Conclusion Curcumin can inhibit the collagen deposition of human embryo lung fibroblasts and play a role in retarding the formation of pulmonary fibrosis. The mechanism may be through inhibiting the expression of TGF-βRⅠ and RⅡ.