逆转录聚合酶链式反应(RT-PCR)分别对119例心肌炎患者,37例正常人血标本进行肠道病毒核糖核酸(RNA)检测,检出率分别为49.6％及5.4％。患者血清柯萨奇B组病毒中和试验阳性率为21.8％。表明RT-PCR是检测肠道病毒感染的一种敏感性高、特异性强、简便快速的方法,优于中和试验。 RT-PCR was performed on 119 viral myocarditis patients and 37 normal controls. The detection rates of enterovirus RNA were 49.6% and 5.4% respectively. The positive rate of patient coxsackie B virus neutralization test was 21.8%. RT-PCR is a sensitive, specific, simple and rapid method for detecting enterovirus infection, which is better than the neutralization test.